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ifferent types of suppressor tRNA (480 mg/mL), and pIPhe (1 mM) to the reaction medium. tRNA denotes synthetic MjtRNACUA, Opt ?tRNACUAOpt. (B) Annotated MS/MS spectrum of the FSVSGEGEGDATY*GK peptide from GFP Y39IPhe. The mass shift between pIPhe and tyrosine was calculated to be 109.9 Da, corresponding to the observed ``y'' ions. doi:10.1371/journal.pone.0068363.gWT GFP or UAA in GFP Y39TAG. The
ifferent types of suppressor tRNA (480 mg/mL), and pIPhe (1 mM) to the reaction medium. tRNA denotes synthetic MjtRNACUA, Opt ?tRNACUAOpt. (B) Annotated MS/MS spectrum of the FSVSGEGEGDATY*GK peptide from GFP Y39IPhe. The mass shift between pIPhe and tyrosine was calculated to be 109.9 Da, corresponding to the observed ``y'' ions. doi:10.1371/journal.pone.0068363.gWT GFP or UAA in GFP Y39TAG. The
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and parasitological confirmation by microscopy. The CATT and trypanolysis tests both rely on immunoglobulins that interact, respectively, with one and three variant antigens on the surface of the trypanosomes; the trypanolysis test is more specific [6]. Microscopy can be supplemented by DNA amplification methods in the unlikely event that facilities are available [2,7]. The only way to determine


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